Antibodies - A LABORATORY MANUAL by Ed Harlow and David Lane

By Ed Harlow and David Lane

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Cold Spring Harbor Symp. Quant. Biol. 41:877-889. , M. Hirama, R. Lenhard-Schuller, and S. Tonegawa. 1978. A complete immunoglobulin gene is created by somatic recombination. Cell15: 114. , K. W. L. Livant, R. L. Weissman, and L. Hood. 1980. An immunoglobulin heavy-chain gene is formed by at least two recombinational events. Nature 283:733-739. , H. Huang, M. Davis, K. Calame, and L. Hood. 1980. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: V", D and J"' Cell19: 981- 992.

The whole interface is tightly packed and excludes solvent. Strikingly, 748 A2 or 11% of the surface of lysozyme is covered by the antibody. Similar conclusions come from the study of the second lysozyme- antibody cocrystals and the neuraminidase-antibody cocrystals. Here, either three (lysozyme) or four (neuraminidase) stretches of distant primary sequence form portions of the epitope structure. Although no cocrystals of epitopes derived from contiguous stretches of amino acids have been solved, work with antibodies raised against synthetic peptides or other small antigens has shown that these interactions exist.

Genet. 9:305-353. Tonegawa, S. 1983. Somatic generation of antibody diversity. Nature 302:575581. J. C. Bennett. 1965. The molecular basis of antibody formation: A paradox. Biochemistry 54:864-869. Hozumi, N. and S. Tonegawa. 1976. Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions. Proc. Natl. Acad. Sci. 73: 3628-3632. , N. Hozumi, G. Matthyssens, and R. Schuller. 1977. Somatic changes in the content and context of immunoglobulin genes. Cold Spring Harbor Symp.

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