By Dr Desmond S. T. Nicholl
Des Nicholl provides a brand new, absolutely revised, and improved version of his renowned undergraduate-level textbook. The publication keeps a few of the gains of the unique version and nonetheless bargains a concise technical creation to the topic of genetic engineering. it's divided into 3 major sections: simple molecular biology, tools of gene manipulation, and glossy functions of genetic engineering. functions lined within the booklet comprise genomics, protein engineering, gene treatment, cloning, transgenic animals and crops, and bioethics. An advent to Genetic Engineering is vital studying for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.
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Extra resources for An Introduction to Genetic Engineering (Studies in Biology)
The actual cutting site of the enzyme will determine the type of ends that the cut fragment has, which is important with regard to further manipulation of the DNA. Three types of fragment may be produced, these being (i) blunt or ﬂush-ended fragments, (ii) fragments with protruding 3Ј ends, and (iii) fragments with protruding 5Ј ends. An example of each type is shown in Fig. 2. Enzymes such as PstI and EcoRI generate DNA fragments with cohesive or ‘sticky’ ends, as the protruding sequences can base-pair with complementary sequences generated by the same enzyme.
2. Digestion of a 15 kb DNA fragment with three restriction enzymes BamHI EcoRI Pstl 14 1 12 3 8 7 BamHI ϩ EcoRI BamHI ϩ PstI EcoRI ϩ PstI 11 3 1 8 6 1 7 5 3 BamHI ϩ EcoRI ϩ PstI 6 5 3 1 Note: Data shown are lengths (in kb) of fragments that are produced on digestion of a 15 kb DNA fragment with the enzymes BamHI, EcoRI and PstI. Single, double and triple digests were carried out as indicated. 3) provide the cutting and joining functions that are essential for the production of recombinant DNA molecules.
2 Polymerases Polymerase enzymes synthesise copies of nucleic acid molecules, and are used in many genetic engineering procedures. When describing a polymerase enzyme, the terms ‘DNA-dependent’ or ‘RNA-dependent’ may be used to 50 The tools of the trade Fig. 5. Mode of action of various nucleases. (a) Nuclease Bal 31 is a complex enzyme. Its primary activity is a fast-acting 3Ј exonuclease, which is coupled with a slow-acting endonuclease. When Bal 31 is present at a high concentration these activities effectively shorten DNA molecules from both termini.